Calprotectin (fecal), BUHLMANN fCAL® ELISA EK-CAL from BUHLMANN Diagnostics Corp

Product Specs
ItemCalprotectin (fecal), BUHLMANN fCAL® ELISA
CompanyBUHLMANN Diagnostics Corp
Price Supplier Page View Company Product Page
Catalog NumberEK-CAL
Quantity1 plate ELISA (96 tests)
ApplicationsELISA
Detection Range30-1800 µg/g
ReactivityHuman
Sample TypeFecal/ Stool extracts
Sensitivity30 µg/g
Detection TargetCalprotectin, MRP8/14, S100A8/A9
Assay Time2.5h (approx.)
Regulatory StatusIVD, FDA Cleared
NCBI Full Gene NameS100 calcium binding protein A8, S100 calcium binding protein A9
NCBI Gene AliasesMA387, L1Ag, MRP14, 60B8AG, CFAG, MRP8, P8, CGLA, NIF, MIF, LIAG, CGLB, P14, MAC387, CAGA, L1AG, CAGB, CP-10
Research AreaPathology/Chemistry/Immunology/Gastroenterology

Add to Compare List

BUHLMANN Diagnostics Corp

105 Route 101A, Suite 1

Amherst, New Hampshire 03031

United States

Phone: (844) 300-9799

Email: info@buhlmannlabs.com

Company Profile

Website: http://buhlmannlabs.com/

(1) An open label, non-randomized study assessing a prebiotic fiber intervention in a small cohort of Parkinson's disease participantsNature communicationsFebruary 18, 2023Hall DA, Voigt RM, Cantu-Jungles TM, Hamaker B, Engen PA, Shaikh M, Raeisi S, Green SJ, Naqib A, Forsyth CB, et al.inflammatory bowel disease (IBD)71. ELISA was used to examine stool calprotectin (BÜHLMANN fCAL® ELISA-EK-CAL; BUHLMANN Diagnostics Corp, Amherst, NH, range: 30–1800 μg/g, samples were run in duplicate according

(2) The Activity of Antimicrobial Peptides in Pediatric Celiac DiseaseFrontiers in PediatricsJune 6, 2022Altinoy T. Kamilova, Gulnoza K. Azizova, Zulkhumar E. Umarnazarova, Dilrabo A. Abdullaeva, Svetlana I. Gellerguidelines (34). Fecal β-defensin-2 (Immundiagnostics, Cat. No.K 6500) and FC levels (Buhlmann, Cat. No. EK-CAL) were measured with an ELISA kit (Immundiagnostics). A one-step sandwich version of solid-phase enzyme immunoassay

(3) Low FODMAP Diet for Functional Gastrointestinal Symptoms in Quiescent Inflammatory Bowel Disease: A Systematic Review of Randomized Controlled TrialsNutrientsNovember 27, 2020Maria G. Grammatikopoulou, Dimitrios G. Goulis, Konstantinos Gkiouras, Meletios P. Nigdelis, Stefanos T. Papageorgiou, Theodora Papamitsou, Alastair Forbes, Dimitrios P. Bogdanossamples Sample fCAL assay: Quantum Blue fCAL (Buhlmann Lab) ELISA ELISA using a commercial kit (Buhlmann EK-Cal, Schönenbuch, Switzerland) Home-administered collecting kit and ELISA Compliance assessment: Dietitian

(4) High-Fat Diet and Antibiotics Cooperatively Impair Mitochondrial Bioenergetics to Trigger Dysbiosis that Exacerbates Pre-inflammatory Bowel DiseaseCell Host & MicrobeAugust 1, 2020Jee-Yon Lee et al.(FCP) analysis. Human FCP level was measured using a commercial enzyme-linked immunosorbent assay kit (EK-CAL, BUHLMANN, Schonenbunch, Switzerland) according to the manufacturer’s instruction. Elevated FCP level

(5) Genetic and Inflammatory Biomarkers Classify Small Intestine Inflammation in Asymptomatic First-degree Relatives of Patients With Crohn’s DiseaseClinical Gastroenterology and HepatologyApril 1, 2020Kirstin M. Taylor, Ken B. Hanscombe, Natalie J. Prescott, Raquel Iniesta, Matthew Traylor, Nicola S. Taylor, Steven Fong, Nicholas Powell, Peter M. Irving, Simon H. Anderson, et al.VCE M AN U and were stored at -80°C before faecal calprotectin (FC) extraction and ELISA analysis (Buhlmann EKCAL Calprotectin, Buhlmann Laboratories, Schönenbuch, Switzerland). Approximately 20ml of blood in serum

(6) Soil‐transmitted helminth infection and intestinal inflammation among the Shuar of Amazonian EcuadorAmerican Journal of Physical AnthropologySeptember 1, 2019Tara J. Cepon‐Robins et al.Amherst, NH) and analyzed using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (EK-CAL; BUHLMANN Diagnostics Corp, Amherst, NH). See Table S1 for assay reliability measures. Fecal calprotectin

(7) A randomised, double-blind, placebo-controlled trial of a multi-strain probiotic in patients with asymptomatic ulcerative colitis and Crohn’s diseaseInflammopharmacologyJune 1, 2019Ingvar Bjarnason, Guy Sission, Bu’Hussaine Hayeeactivity scores between active and placebo treatment and changes in laboratory measures including FCAL (EK-CAL, Buhlmann, Switzerland). Statistics All statistical analyses was carried out by using SAS Software

(8) Consecutive fecal calprotectin measurements for predicting relapse in pediatric Crohn’s disease patientsWorld Journal of GastroenterologyMarch 14, 2019Alice Jane Foster, Matthew Smyth, Alam Lakhani, Benjamin Jung, Rollin F Brant, Kevan JacobsonManual ELISA was performed within 4 mo of sample extraction using the Buhlmann fCAL® ELISA kit (Buhlmann EK-CAL, rev 2012-11-20) following vendor’s instructions for the lower range procedure (working 10-600 μg/g).

(9) Evaluation of the clinical and cost-effectiveness of the York Faecal Calprotectin Care PathwayFrontline GastroenterologyOctober 1, 2018James Turvill, Daniel Turnock, Hayden Holmes, Alison Jones, Eleanor Mclaughlan, Victoria Hilton, Stacey Marriottat 4°C, each stool sample was extracted and analysed using Buhlmann CALEX extraction tubes and the EK-CAL Calprotectin ELISA, respectively (supplied by Alpha Laboratories) to determine the FC level at a cost

(10) Diagnostic accuracy of fecal calprotectin in assessing the severity of inflammatory bowel disease: From laboratory to clinicCaspian J Intern MedSeptember 22, 2017Soheila Moein, Durdi Qujeq, Mostafa Vaghari Tabari, Mehrdad Kashifard, Karimollah Hajian-Tilakimeasured by ELISA sandwich test according to kit’s instructions (Buhlmann Co, Switzerland, cat number .EK-CAL), the kit suggested a cut-off level of <50µg/g. CRP was measured by Auto Analyzer (Hitachi Co. Japan)

(11) Validation of a care pathway for the use of faecal calprotectin in monitoring patients with Crohn's diseaseFrontline GastroenterologyJuly 1, 2017James Turvill, Lisa Rook, Maxine Rawle, Gerry Robins, Simon Smale, Prashant Kant, Anne Phillipsstored at −20° C and then analysed in batches. The stool extract was analysed using a monoclonal ELISA (EK-CAL calprotectin ELISA, Buhlmann, supplied by Alpha Laboratories) to determine the FC level. The normal cut-off

(12) Calprotectin in Daily Practice: Where Do We Stand in 2017?DigestionFebruary 2, 2017Fabrizia D'Angelo, Christian Felley, Jean Louis Frossardreading) Certest extraction device 50–200 PhiCal test (Oslo, Norway) ELISA Calpro extraction device 50–500 EK-CAL (Buhlmann, Schonenbuch, ELISA Switzerland) Roche faecal extraction device 20–2,000 Prevent ID caldetect

(13) FECAL CALPROTECTIN AND MICROBIOTA OF THE GUTБюллетень Восточно-Сибирского научного центра Сибирского отделения Российской академии медицинских наукNovember 28, 2016Богородская, Svetlana Bogorodskaya, Чашкова, Elena Chashkova, Горохова, Viktoriya Gorokhova, Кузнецова, Emma Kuznetsova, Пак, Vyacheslav Pakпроизводителей на основе поликлональных антител PhiCal (производитель Calpro AS, Норвегия) и моноклональных антител EK-CAL (Buhlmann Laboratories, Швейцария). Лучший результат показал набор на основе моноклональных антител.

(14) Evaluation of a faecal calprotectin care pathway for use in primary carePrimary Health Care Research & DevelopmentSeptember 1, 2016James Turvill, Shaun O’Connell, Abigail Brooks, Karen Bradley-Wood, James Laing, Swaminathan Thiagarajan, David Hammond, Daniel Turnock, Alison Jones, Ruchit Sood, et al.it within 24 h. The stool sample was processed using a monoclonal enzyme-linked immunosorbent assay (EK-CAL Calprotectin ELISA, Buhlmann; Alpha Laboratories Ltd, Eastleigh, Hants, UK) to determine the FC level.

(15) Consistent Prebiotic Effect on Gut Microbiota With Altered FODMAP Intake in Patients with Crohn's Disease: A Randomised, Controlled Cross-Over Trial of Well-Defined DietsClinical and Translational GastroenterologyApril 14, 2016Emma P Halmos, Claus T Christophersen, Anthony R Bird, Susan J Shepherd, Jane G Muir, Peter R Gibsonthe content of calprotectin. This was assessed in duplicate by ELISA using a commercial kit (Buhlmann EK-Cal, Schönenbuch, Switzerland) as per manufacturer's instructions. The results were expressed as an average

(16) Are faecal markers good indicators of mucosal healing in inflammatory bowel disease?World Journal of GastroenterologyMay 8, 2015Gudula JAM Boon, Andrew S Day, Chris J Mulder, Richard B Gearryscoring system[63]; Cal: Calpro ELISA: Calpro Calprotectin ELISA, Calpro AS, Norway; BM: BM ELISA, EK-CAL, Buhlmann Laboratories AG, Switzerland; Farmer: Non-standard endoscopic scoring system[64]; Faecal Hb:

(17) PMO-244 Infliximab reduces the need for correction of fistulae and drainage of abscesses: a UK retrospective study of Crohn's disease patientsGutJuly 1, 2012C D Wheeler, R Chipperfield, T Orchard, J Lindsayextraction of native stool, was assessed at a range of clinically relevant concentrations: Buhlmann EK-CAL 17% (10 mg/g), 12% (47 mg/g), 19% (62 mg/g); PhiCal1 21% (8 mg/g), 24% (10 mg/g), 18% (27 mg/g); PhiCal2

(18) PMO-241 Conveying medication benefits to ulcerative colitis patients: what thresholds for adherence are applied?GutJuly 1, 2012C Selinger, Y Kinjo, J McLaughlin, A Robinson, R Leongresults were compared. FC was measured by ELISA assays: Immundiagnostik PhiCal (version 1) and Buhlmann EK-CAL. A subset were also measured by PhiCal (version 2). Stool was weighed and extracted, and ELISAs performed manually. Results

(19) PMO-242 The IBD-control questionnaire: development and psychometric validation of a tool for capturing disease control from the patient perspective for use in routine careGutJuly 1, 2012C Ormerod, D Shackcloth, M Harrison, E Brown, K Bodgerresults were compared. FC was measured by ELISA assays: Immundiagnostik PhiCal (version 1) and Buhlmann EK-CAL. A subset were also measured by PhiCal (version 2). Stool was weighed and extracted, and ELISAs performed manually. Results

(20) PMO-245 Infliximab reduces rates of emergency hospital admissions and surgical procedures: a UK retrospective study of Crohn's disease patientsGutJuly 1, 2012C D Wheeler, R Chipperfield, T Orchard, J Lindsayextraction of native stool, was assessed at a range of clinically relevant concentrations: Buhlmann EK-CAL 17% (10 mg/g), 12% (47 mg/g), 19% (62 mg/g); PhiCal1 21% (8 mg/g), 24% (10 mg/g), 18% (27 mg/g); PhiCal2

Be the First to Write a Review!

Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Calprotectin (fecal), BUHLMANN fCAL® ELISA from BUHLMANN Diagnostics Corp

Description

BUHLMANN Diagnostics Corp

Citations (23)

Reviews

Bench Tips

Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Calprotectin (fecal), BUHLMANN fCAL® ELISA from BUHLMANN Diagnostics Corp

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map